Title | Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus. |
Publication Type | Journal Article |
Year of Publication | 2010 |
Authors | Liu, X, Zhang, Q, Murata, K, Baker, ML, Sullivan, MB, Fu, C, Dougherty, MT, Schmid, MF, Osburne, MS, Chisholm, SW, Chiu, W |
Journal | Nat Struct Mol Biol |
Volume | 17 |
Issue | 7 |
Pagination | 830-6 |
Date Published | 2010 Jul |
ISSN | 1545-9985 |
Keywords | Capsid, Genome, Viral, Models, Molecular, Podoviridae, Prochlorococcus, Viral Proteins, Virion |
Abstract | Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single-particle cryo-electron microscopy yields icosahedral and asymmetrical structures of infectious P-SSP7 with 4.6-A and 9-A resolution, respectively. The asymmetric reconstruction reveals how symmetry mismatches are accommodated among five of the gene products at the portal vertex. Reconstructions of infectious and empty particles show a conformational change of the 'valve' density in the nozzle, an orientation difference in the tail fibers, a disordering of the C terminus of the portal protein and the disappearance of the core proteins. In addition, cryo-electron tomography of P-SSP7 infecting Prochlorococcus showed the same tail-fiber conformation as that in empty particles. Our observations suggest a mechanism whereby, upon binding to the host cell, the tail fibers induce a cascade of structural alterations of the portal vertex complex that triggers DNA release. |
DOI | 10.1038/nsmb.1823 |
Alternate Journal | Nat. Struct. Mol. Biol. |
PubMed ID | 20543830 |
PubMed Central ID | PMC2924429 |
Grant List | P41 RR002250-23 / RR / NCRR NIH HHS / United States P41 RR002250-237254 / RR / NCRR NIH HHS / United States P41 RR002250-24 / RR / NCRR NIH HHS / United States P41 RR002250-247897 / RR / NCRR NIH HHS / United States P41 RR002250-25 / RR / NCRR NIH HHS / United States P41 RR008605-158419 / RR / NCRR NIH HHS / United States P41 RR008605-165471 / RR / NCRR NIH HHS / United States P41RR002250 / RR / NCRR NIH HHS / United States R01 AI075208-01A1 / AI / NIAID NIH HHS / United States R01 AI075208-02 / AI / NIAID NIH HHS / United States R01 GM079429-02 / GM / NIGMS NIH HHS / United States R01 GM079429-03 / GM / NIGMS NIH HHS / United States R01 GM079429-05 / GM / NIGMS NIH HHS / United States R01GM079429 / GM / NIGMS NIH HHS / United States |