Culturing Prochlorococcus, Synechococcus, SAR11, and marine heterotrophic bacteria

Described in this manual are commonly used protocols and media recipes used to maintain Prochlorococcus, Synechococcus, SAR11, and marine heterotrophic bacteria. Descriptions of the intricacies of plating both Prochlorococcus and Synechococcus, the measures taken to remove metals from cultureware, suggestions for media and bottle modification for growing Prochlorococcus in large volumes (1-20L), descriptions of methods to cryopreserve  for long-term storage and shipment are provided. Also discussed are methods for preserving samples for flow cytometry, how cultures are measured, how growth rates are calculated, and what incubators and tools are used to maintain and monitor cultures. Finally, tips for successfully growing Prochlorococcus and Synechococcus are described.

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