Marcia S. Osburne

Research Scientist
Civil and Environmental Engineering
MIT 48-321
15 Vassar Street
Cambridge, MA 02139
Phone: (617) 253-3310
Fax: (617) 258-7009
Email: mosburne at mit.edu

Education:

1971 B.S. Biology. Massachusetts Institute of Technology
1977 Ph.D. Molecular Biology and Microbiology ,Tufts University

Publications:

Tummala S, Sheth SG, Goldsmith JD, Goldar- Najafi A, Murphy CK, Osburne MS, Mullin S, Buxton D, Wagner DA, and Kelly C.2007
Quantifying gastric Helicobacter pylori infection: a comparison of quantitative culture, urease breath testing and histology.
Dig. Dis. and Sciences, 52:396-401. Epub 2007 Jan 12..

Osburne, M. S., Murphy, C.K., and Rothstein, D.M. 2006.
Enhanced activity of Rifalazil in combination with Levofloxacin, Linezolid, or Mupirocin against Staphylococcus aureus in in vitro time-kill studies.
J. Antibiotics. 59, 303-308.

Osburne MS, Rothstein DM, Farquhar R, Murphy CK 2006.
In Vitro Time-kill Activities of Rifalazil, Alone and in Combination with Vancomycin, against Logarithmic and Stationary Cultures of Staphylococcus aureus
J. Antibiotics 59, 80-85

Murphy CK, Mullin S, Osburne MS, van Duzer J, Siedlecki J, Yu X, Kerstein K, Cynamon M, Rothstein DM. 2006.
In Vitro Activity of Novel Rifamycins against Rifamycin-Resistant Staphylococcus aureus
Antimicrob Agents Chemother. 50(3):827-34.

Martinez, A., Hopke, J., MacNeil I.A., and Osburne, M.S. 2005
Accessing the genomes of uncultivated microbes for novel natural products.
Natural Products: Drug Discovery, Therapeutics and Preventive Medicine.
Humana Press, Totowa, NJ, pp. 295-312.

Martinez, A., Kolvek, S., Jopke, J., and Osburne M.S. 2005
An environmental DNA sequence conferring early and increased sporulation and antibiotic production in Streptomyces lividans.
Appl. and Environ. Micro. 71, 1638-41.

Martinez, A., Kolvek, S.J., Yip, C.L., Hopke, J., Brown, K., MacNeil, I.A., and Osburne, M.S. 2004
Genetically modified bacterial strains and novel bacterial artificial chromosome shuttle vectors for constructing environmental libraries and detecting heterologous natural products in multiple expression hosts.
Appl. and Environ. Micro. 70, 2452-2463.

Courtois, S., Cappellano, C., Ball, M., Francou, F., Normand, F., Helynck, G., Martinez, A., Kolvek, S., Hopke, J., Osburne, M.S., August, P., Nalin, R., Guerineau, M., Jeannin, P., Simonet, P., and Perdonet, J.L. 2003
Recombinant environmental libraries provide access to microbial diversity for natural products drug discovery.
Appl. and Environ. Micro. 69, 49-55.

MacNeil, I.A., Tiong, C. L., Minor C., August, P.R., Grossman T.H., Loiacono, K.A., Lynch B.A., Tyler, A., Phillips, T. Call, Aldrich, T., Long, H., Dalgarno, D., Narula, S., Sundaramoorthi, Gilman, M., Holt, D., Osburne M.S. 2001
Expression and Isolation of Antimicrobial Small Molecules from Soil DNA Libraries.
J. Mol. Micro.and Biotechnology 3, 301-308.

Osburne, M. S., Grossman, T. H., August, P.R., and I. A. MacNeil. 2000
Tapping into microbial diversity for natural products drug discovery - a new approach.
ASM News 66, 411-417.

Rondon, M. R., August, P. A., Bettermann, A. D., Brady, S. F., Grossman, T. H., Liles, M. R., Loiacono, K., Lynch, B. A., MacNeil, I. A., Minor, C., Tiong, C. L., Gilman, M., Osburne, M. S., Clardy, J., Handelsman, J., and R. M. Goodman. 2000
Cloning the soil metagenome: a Strategy for Accessing the Genetic and Functional Diversity of Uncultured Microorganisms.
Appl. Environ. Micro. 66, 2541-2547.

August, P.R., T.H. Grossman, C. Minor, M.P. Draper, I.A. MacNeil, K. Call, D. Holt, and M. S. Osburne. 2000
Sequence analysis and functional characterization of the violacein biosynthetic pathway from Chromobacterium violaceium.
Journal of Molecular Microbiology and Biotechnology 2, 513-519.

Osburne, Marcia S., Neidhardt, Edith A., Godoy, Jorge E., van Schravendijk, Marie R., and Trudy H. Grossman. 1999
Production of secreted, soluble human two-domain CD4 protein in Escherichia coli..
J. Immunol. Methods 224, 19-24.

Hare, Brian J., Wyss, Daniel F., Osburne, Marcia S., Kern, Petra, Reinherz, Ellis L., and Gerhard Wagner. 1999
Structure, specificity, and CDR mobility of a class II restricted single-chain T cell receptor.
Nature Structural Biology 6, 574-581.

Golden, Alex, Austen, Douglas A., van Schravendijk, Marie R., Sullivan, Barbara. J., Kawasaki, Ernest. S., and Marcia S. Osburne. 1998
Effect of Promoters and Signal Sequences on the Production of Secreted HIV-1 gp120 Protein in the Baculovirus System.
Protein Expr. Purif. 14, 8-12.

Grossman, Trudy H., Ernest S. Kawasaki, Sandhya R. Punreddy, and Marcia S. Osburne. 1998
Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability.
Gene 209, 95-103.

Ferrer, Marc, Kevin L. Godbout, Barbara J. Sullivan, Douglas A. Austen, Christine T. Sanderson, Kevin C. Kelley, Marcia S. Osburne, Stephen C. Harrison, and Marie R. van Schravendijk. 1997
Construction and characterization of a radio-iodinatable mutant of recombinant human CD4.
J. Immunol. Methods 210, 215-225.

Khandekar, Sanjay S., Brian M. Bettencourt, Daniel F. Wyss, Jerome W. Naylor, Pamela P. Brauer, Kevin Heustis, Donard S. Dwyer, Albert T. Profy, Marcia S. Osburne, Julian Banerji, and Barry Jones. 1997
Conformational Integrity and Ligand Binding Properties of a Single Chain T-Cell Receptor Expressed in Escherichia coli.
J. Biol. Chem., 272, 32190-32197.

Golden, Alex, Sanjay S. Khandekhar, Marcia S. Osburne, Ernest S. Kawasaki, Ellis L. Reinherz, and Trudy H. Grossman.1997
High-level production of a secreted, heterodimeric aß murine T-cell receptor in Escherichia coli.
J. Immunol. Meths. 206, 163-169.

Grossman, Trudy H., Jorge E. Godoy, Ernest S. Kawasaki, and Marcia S. Osburne,1997
A novel mammalian expression vector for the rapid identification of high-producing stable clones.
Plasmid 37, 155-158.

Rowlands, Belinda, Trudy Grossman, Marcia Osburne, and Harry Tabor1996
Sequence and genetic organization of a Bacillus subtilis operon encoding 2,3 dihydroxybenzoate biosynthetic enzymes.
Gene 178, 119-123.

Rusconi, Stefano, Mona Moonis, Debra P. Merrill, Peter V. Pallai, Edith A. Neidhardt, Shyam K. Singh, Kevin J. Willis, Marcia S. Osburne, Albert T. Profy, James C. Jenson, and Martin S. Hirsch, 1996
Naphthalene sulfonate condensates with CD4-blocking and anti-HIV-1 activity.
Antimicrob. Ag. and Chemother. 40, 234-236.

Osburne, Marcia S. 1995
Characterization of a cinodine-resistant mutant of Escherichia coli.
J. Antibiot. 48, 1359-1361.

Kelley, Kevin C., Kevin J. Huestis, Douglas A. Austen, Christine T. Sanderson, Mary A. Donoghue, Shawn K. Stickel, Ernest S. Kawasaki, and Marcia S. Osburne, 1995
Regulation of sCD4-183 gene expression from T7-based vectors in Escherichia coli.
Gene 156, 33-36.

Profy, A.T., van Schravendijk, M.R., Willis, K.J., Pallai, P.V., Neidhardt, E.A., Singh, S.K., Wu, J., Gordon, K., Sampo, T.M., Osburne, M.S., Sonderfan, A.J., Wortel, C.H., Rusconi, S., Merrill, D.P., Moonis, M., Hirsch, M.S., Buckheit, R.W., and Jenson, J. C. Jr., 1995
PRO 2000: a new antiviral compound that blocks gp120 binding to CD4.
Proceedings of the Tenth Colloque des Cent Gardes on “Retroviruses of huuman AIDS and releated human diseases,” Marnes-la-Coquette, France, October 23-25, 1995. pp. 165-169.

Tuckman, Margareta K., Ian Chopra, and Marcia S. Osburne, 1994
An assay to detect inhibitors of bacterial iron transport.
J. Antibiot. 47, 123-125.

Grossman, Trudy H., Margareta Tuckman, Sarah Ellestad, and Marcia S. Osburne, 1993
Isolation and characterization of Bacillus subtilis genes involved in siderophore biosynthesis; relationship between B. subtilis sfp0 and E. coli entD genes.
J. Bacteriol. 175, 6203-6211.

Osburne, Marcia S., William M. Maiese and Michael Greenstein, 1993
An assay for the detection of bacterial DNA gyrase inhibitors.
J. Antibiot. 46, 1764-1766.

Tuckman, Margareta, and Marcia S. Osburne. 1992
In vivo inhibition of TonB-dependent processes by a TonB box consensus pentapeptide.
J. Bacteriol. 174: 320-323

Osburne, Marcia S., Susan M. Zavodny, Michael Greenstein, and William M. Maiese, 1992
Phenotypes conferred by the Bacillus subtilis recM13 mutation and the din23 fusion.
Mutation Research, DNA Repair, 274: 79-84.

Osburne, Marcia S., William M. Maiese, and Michael Greenstein, 1990
In vitro inhibition of bacterial DNA gyrase by cinodine, a glycocinnamoylspermidine antibiotic.
Antimicrob. Ag.Chemother. 34: 1450-1452.

Osburne, Marcia S., Susan M. Zavodny, and Gail A. Peterson, 1988
Drug-induced relaxation of supercoiled plasmid DNA in B. subtilis and induction of the SOS response.
J. Bacteriol. 170: 442-445.

Osburne, Marcia S., and R. Craig, 1986
Activity of two strong promoters cloned into Bacillus subtilis.
J. Gen Microbiol. 132: 565-568.

Osburne, Marcia S., R. Craig, and D. M. Rothstein. 1985
Thermoinducible transcription system for Bacillus subtilis that utilizes control elements from temperate phage ø105.
J. Bacteriol. 163: 1101-1108.

Osburne, Marcia S., 1982
Rhizobium meliloti mutants altered in ammonium utilization.
J. Bacteriol. 151: 1633-1636.

Osburne, Marcia S., and E.R. Signer.. 1980
Ammonium assimilation in Rhizobium meliloti.
J. Bacteriol. 143: 1234-1240.

Osburne, Marcia S., and A.L. Sonenshein, 1980
Inhibition by lipiarmycin of phage growth in Bacillus subtilis.
J. Virol. 33:945-953.

Osburne, Marcia S., and A. L. Sonenshein, 1976
Behavior of a temperate phage in differentiating cells of Bacillus subtilis.
J. Virol. 19: 26-35.